The DNMT1 Polyclonal Antibody (CAB16833) is specifically designed for research involving DNMT1, a key enzyme involved in DNA methylation and gene regulation. This rabbit polyclonal antibody is highly reactive with human samples and has been validated for use in Western blot applications. By binding to the DNMT1 protein, this antibody enables precise detection and analysis in various cell types, making it an essential tool for studies in epigenetics and cancer research.
DNMT1, also known as DNA methyltransferase 1, plays a crucial role in maintaining DNA methylation patterns during cell division and development. Aberrant DNMT1 activity has been linked to various diseases, including cancer and neurological disorders. Understanding the function of DNMT1 is essential for unraveling the mechanisms of gene regulation and disease development, thereby paving the way for potential therapeutic interventions targeting epigenetic modifications.
Product Name:
DNMT1 Rabbit Polyclonal Antibody
SKU:
CAB16833
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-124 of DNMT1 (NP_001370.1).
This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
1786
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using DNMT1 Rabbit pAb (CAB16833) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
