The DNMT1 Polyclonal Antibody (CAB5495) is a highly specific research tool designed for studying DNA methyltransferase 1 (DNMT1), an enzyme responsible for maintaining DNA methylation patterns during cell division. This antibody is raised in rabbits and is validated for use in various applications, including Western blot and immunohistochemistry.DNMT1 plays a crucial role in epigenetic regulation by adding methyl groups to DNA, which can affect gene expression and cellular differentiation. Dysregulation of DNMT1 has been linked to various diseases, including cancer, neurological disorders, and developmental abnormalities.
Therefore, studying the expression and activity of DNMT1 is essential for understanding its role in disease pathogenesis and developing targeted therapies.This antibody offers high specificity and sensitivity in detecting DNMT1 protein levels in samples, making it a valuable tool for researchers in the fields of epigenetics, oncology, and developmental biology. Its versatility and reliability make it an ideal choice for studies aiming to unravel the complex mechanisms underlying DNA methylation and its impact on cellular processes.
Product Name:
[KO Validated] DNMT1 Rabbit Polyclonal Antibody
SKU:
CAB5495
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 760-1109 of DNMT1 (NP_001370.1).
This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
1786
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Immunoprecipitation analysis of 200 μg extracts of 293T cells using 3 μg DNMT1 antibody (CAB5495). Western blot was performed from the immunoprecipitate using DNMT1 antibody (CAB5495) at a dilution of 1:1000.
