The DNMT1 Polyclonal Antibody (CAB21378) is specifically designed for research involving DNA methyltransferase 1 (DNMT1), a key enzyme involved in DNA methylation and gene expression regulation. This antibody, raised in rabbits, is highly reactive with human samples and has been validated for use in Western blot applications. By binding to the DNMT1 protein, this antibody enables researchers to detect and analyze DNMT1 expression in various cell types, making it an ideal tool for studies in epigenetics, cancer research, and developmental biology.DNMT1 is known for its essential role in maintaining DNA methylation patterns during cell division, which is crucial for gene silencing and genomic stability.
Dysregulation of DNMT1 has been linked to various disease conditions, including cancer, neurodevelopmental disorders, and cardiovascular diseases. Understanding the function and expression of DNMT1 is vital for unraveling its impact on disease pathology and for developing targeted therapies that can modulate DNA methylation patterns.Overall, the DNMT1 Polyclonal Antibody (CAB21378) offers researchers a reliable tool for investigating the role of DNMT1 in epigenetic regulation and disease processes, ultimately advancing our understanding of the molecular mechanisms underlying complex diseases and paving the way for novel therapeutic strategies.
Product Name:
DNMT1 Polyclonal Antibody
SKU:
CAB21378
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1417-1616 of human DNMT1 (NP_001370.1).
This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
1786
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using DNMT1 Rabbit pAb (CAB21378) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
