DNMT1 Polyclonal Antibody (CAB21378)
- SKU:
- CAB21378
- Product Type:
- Antibody
- Antibody Type:
- Polyclonal Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- WB
- IF
- IP
Frequently bought together:
Description
| Product Name: | DNMT1 Polyclonal Antibody |
| SKU: | CAB21378 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1417-1616 of human DNMT1 (NP_001370.1). |
| Sequence: | MSAL VAAR MRHI PLAP GSDW RDLP NIEV RLSD GTMA RKLR YTHH DRKN GRSS SGAL RGVC SCVE AGKA CDPA ARQF NTLI PWCL PHTG NRHN HWAG LYGR LEWD GFFS TTVT NPEP MGKQ GRVL HPEQ HRVV SVRE CARS QGFP DTYR LFGN ILDK HRQV GNAV PPPL AKAI GLEI KLCM LAKA RESA SAKI KEEE AAKD |
| Tested Applications: | WB IF/ICC IP ChIP ELISA |
| Recommended Dilution: | WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cellsChIP,3μg antibody for 10μg-15μg of Chromatin |
| Synonyms: | AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN; m.HsaI; DNMT1 |
| Positive Sample: | 293T,SW480,Jurkat |
| Conjugate: | Unconjugated |
| Cellular Localization: | Nucleus. |
| Calculated MW: | 183kDa |
| Observed MW: | Refer to figures |
This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants.
| Purification Method: | Affinity purification |
| Gene ID: | 1786 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
 | Western blot analysis of various lysates using DNMT1 Rabbit pAb (CAB21378) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s. |
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