The DNMT1 Polyclonal Antibody (CAB16729) is a valuable tool for research involving DNA methyltransferase 1 (DNMT1), a key enzyme involved in DNA methylation and gene regulation. This antibody, raised in rabbits, is highly specific and reactive with human samples, making it ideal for use in various experimental techniques including Western blotting.DNMT1 plays a crucial role in maintaining DNA methylation patterns during cell division and differentiation, and dysregulation of this enzyme has been implicated in various diseases including cancer and neurodevelopmental disorders. The DNMT1 Polyclonal Antibody enables researchers to detect and analyze DNMT1 expression levels in different cell types, allowing for a better understanding of its function in health and disease.
By studying DNMT1 expression and activity using this antibody, researchers can gain insights into epigenetic mechanisms of gene regulation and potentially identify targets for therapeutic interventions in conditions where DNA methylation plays a role. The DNMT1 Polyclonal Antibody is a valuable tool for scientists working in the fields of epigenetics, cancer research, and developmental biology.
Product Name:
DNMT1 Rabbit Polyclonal Antibody
SKU:
CAB16729
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-124 of DNMT1 (NP_001370.1).
This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
1786
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and DNMT1 knockdown (KD) 293T cells using [KD Validated] DNMT1 Rabbit pAb (CAB16729) at 1:1000 dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L)(CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020)Exposure time: 3 s.
