The DNAse2 Polyclonal Antibody (PACO49530) is a research tool designed for studying DNAse2, an enzyme involved in the degradation of DNA in cells. This antibody is raised in rabbits and is highly reactive with human samples, making it a valuable tool for researchers working in the field of molecular biology.DNAse2 is known for its role in DNA degradation, which is essential for various cellular processes such as apoptosis, DNA repair, and chromatin remodeling. The DNAse2 Polyclonal Antibody binds specifically to DNAse2 protein, allowing for detection and analysis in a wide range of cell types.
This antibody is validated for use in Western blot applications, providing researchers with a reliable tool for studying DNAse2 expression and function.Studying DNAse2 is crucial for understanding its role in cellular processes and its potential implications in diseases such as cancer, autoimmune disorders, and genetic disorders. The DNAse2 Polyclonal Antibody offers researchers a powerful tool for unraveling the complex mechanisms involving DNA degradation and its impact on various biological processes.
Western Blot. Positive WB detected in: MCF-7 whole cell lysate, PC-3 whole cell lysate. All lanes: DNASE2 antibody at 2.7µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 40, 34 kDa. Observed band size: 40 kDa..
IHC image of PACO49530 diluted at 1:400 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Hydrolyzes DNA under acid, c conditions with a preference for double-stranded DNA. Plays a major role in the degradation of nuclear DNA in cellular apoptosis during development. Necessary for proper fetal development and for definitive erythropoiesis in fetal liver, where it degrades nuclear DNA expelled from erythroid precursor cells.
Synonyms:
Deoxyribonuclease-2-α (EC 3.1.22.1) (acid, DNase) (Deoxyribonuclease II alpha) (DNase II alpha) (Lysosomal DNase II) (R31240_2), DNASE2, DNASE2A DNL2
UniProt Protein Function:
DNASE2: Hydrolyzes DNA under acidic conditions with a preference for double-stranded DNA. Plays a major role in the degradation of nuclear DNA in cellular apoptosis during development. Necessary for proper fetal development and for definitive erythropoiesis in fetal liver, where it degrades nuclear DNA expelled from erythroid precursor cells. Belongs to the DNase II family.Protein type: EC 3.1.22.1; DeoxyribonucleaseChromosomal Location of Human Ortholog: 19p13.2Cellular Component: lysosome; intracellularMolecular Function: DNA binding; deoxyribonuclease II activityBiological Process: erythrocyte differentiation; DNA fragmentation during apoptosis; DNA metabolic process
UniProt Protein Details:
NCBI Summary:
This gene encodes a member of the DNase family. The protein, located in the lysosome, hydrolyzes DNA under acidic conditions and mediates the breakdown of DNA during erythropoiesis and apoptosis. Two codominant alleles have been characterized, DNASE2*L (low activity) and DNASE2*H (high activity), that differ at one nucleotide in the promoter region. The DNASE2*H allele is represented in this record. [provided by RefSeq, Jul 2008]
