The DNA Ligase I Polyclonal Antibody (CAB8267) is a vital tool for researchers studying DNA repair mechanisms and processes involved in maintaining genomic stability. This antibody, produced in rabbits, exhibits high specificity and sensitivity for detecting the DNA Ligase I protein in human samples, making it an essential component for experiments utilizing Western blot techniques.DNA Ligase I is an enzyme critical for sealing breaks in DNA strands during replication and repair, ensuring the integrity of the genetic material.
Dysregulation or mutations in the DNA Ligase I gene have been associated with various diseases, including cancer and genetic disorders. By utilizing the DNA Ligase I Polyclonal Antibody, researchers can investigate the expression and function of this enzyme in different cell types and conditions, providing valuable insights into disease mechanisms and potential therapeutic targets.
Product Name:
DNAL1 Rabbit Polyclonal Antibody
SKU:
CAB8267
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 41-190 of human DNAL1 (NP_113615.2).
This gene encodes an axonemal dynein light chain which functions as a component of the outer dynein arms complex. This complex acts as the molecular motor that provides the force to move cilia in an ATP-dependent manner. The encoded protein is expressed in tissues with motile cilia or flagella and may be involved in the movement of sperm flagella. Alternate splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
83544
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of mouse testis, using DNAL1 antibody (CAB8267) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
