The DNA Ligase I Polyclonal Antibody (CAB12601) is a vital tool for research involving DNA ligase I, an essential enzyme responsible for joining DNA strands together during DNA replication and repair processes. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, making it ideal for use in Western blot applications. By binding to the DNA ligase I protein, this antibody facilitates the detection and analysis of this critical enzyme in a variety of cell types, making it valuable for studies in genetics, molecular biology, and cancer research.
DNA ligase I is a key player in maintaining genomic stability and integrity by sealing nicks in the DNA backbone and facilitating the repair of DNA damage. Its crucial role in DNA metabolism makes it a relevant target for research in various genetic disorders, cancer development, and therapeutic strategies. Understanding the function and regulation of DNA ligase I is essential for unraveling the complexities of DNA repair mechanisms and developing treatments for diseases linked to genome instability.
Product Name:
DNAL1 Rabbit Polyclonal Antibody
SKU:
CAB12601
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 41-190 of human DNAL1 (NP_113615.2).
This gene encodes an axonemal dynein light chain which functions as a component of the outer dynein arms complex. This complex acts as the molecular motor that provides the force to move cilia in an ATP-dependent manner. The encoded protein is expressed in tissues with motile cilia or flagella and may be involved in the movement of sperm flagella. Alternate splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
83544
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using DNAL1 Antibody (CAB12601) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
