The DNAJC6 Polyclonal Antibody (PAC02673) is a valuable tool for researchers studying the DNAJC6 protein, which is involved in various cellular processes including protein folding and trafficking. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.The DNAJC6 protein, also known as auxilin, is a key player in the uncoating of clathrin-coated vesicles, a process essential for intracellular vesicle trafficking. Dysregulation of DNAJC6 has been linked to neurodegenerative disorders such as Parkinson's disease, making it a promising target for therapeutic interventions.
By utilizing the DNAJC6 Polyclonal Antibody, researchers can effectively detect and analyze the DNAJC6 protein in various cell types, providing valuable insights into its function and potential implications in disease pathology. This antibody is an essential tool for studies in cell biology, neurology, and neurodegenerative diseases, offering new opportunities for understanding the molecular mechanisms underlying these conditions.
Immunohistochemistry of paraffin-embedded human brain tissue using PACO26273 at dilution of 1:100.
Immunofluorescent analysis of Hela cells using PACO26273 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunohistochemistry of paraffin-embedded human skin tissue using PACO26273 at dilution of 1:100.
Background:
Recruits HSPA8/HSC70 to clathrin-coated vesicles and promotes uncoating of clathrin-coated vesicles. Plays a role in clathrin-mediated endocytosis in neurons.
Synonyms:
Putative tyrosine-protein phosphatase auxilin (EC 3.1.3.48) (DnaJ homolog subfamily C member 6), DNAJC6, KIAA0473
UniProt Protein Function:
DNAJC6: Recruits HSPA8/HSC70 to clathrin-coated vesicles and promotes uncoating of clathrin-coated vesicles. 3 isoforms of the human protein are produced by alternative splicing.Protein type: Motility/polarity/chemotaxis; Protein phosphatase, tyrosine (non-receptor); Chaperone; EC 3.1.3.48Chromosomal Location of Human Ortholog: 1p31.3Cellular Component: cytosolDisease: Parkinson Disease 19, Juvenile-onset
UniProt Protein Details:
NCBI Summary:
DNAJC6 belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus, a glycine/phenylalanine (G/F)-rich region, and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain (Ohtsuka and Hata, 2000 [PubMed 11147971]).[supplied by OMIM, Mar 2008]
