The DNAJC4 Antibody (PAC053166) is a polyclonal antibody designed for research involving DNAJC4, a member of the DNAJ/HSP40 family of proteins involved in chaperone activity and protein folding. This antibody, raised in rabbits, is highly specific to human DNAJC4 and validated for use in various applications such as Western blot and immunofluorescence.DNAJC4 is known to interact with HSP70, playing a crucial role in protein folding and quality control of misfolded proteins. It is implicated in various cellular processes, including DNA damage repair, apoptosis, and cell proliferation. This antibody binds specifically to DNAJC4, allowing for its detection and analysis in different cell types, making it an ideal tool for studies in molecular biology and cell signaling research.
Understanding the function of DNAJC4 is important for unraveling its role in protein folding and cellular homeostasis. Its involvement in key cellular processes makes it a promising target for research into neurodegenerative diseases, cancer, and other conditions associated with protein misfolding. By studying DNAJC4 and its interactions, researchers can gain insights into potential therapeutic strategies targeting protein folding and quality control mechanisms.
Western Blot. Positive WB detected in: Mouse brain tissue. All lanes: DNAJC4 antibody at 3.2µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 28 kDa. Observed band size: 28 kDa.
Immunofluorescent analysis of A549 cells using PACO53166 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunohistochemistry of paraffin-embedded human colon cancer using PACO53166 at dilution of 1:100.
Background:
membrane, unfolded protein binding, protein folding, response to unfolded protein
Synonyms:
DnaJ homolog subfamily C member 4 (DnaJ-like protein HSPF2) (Multiple endocrine neoplasia type 1 candidate protein number 18), DNAJC4, HSPF2 MCG18
UniProt Protein Function:
DNAJC4: Protein type: Chaperone; Membrane protein, integral; MitochondrialChromosomal Location of Human Ortholog: 11q13.1Cellular Component: integral component of membrane; membraneMolecular Function: protein binding; unfolded protein bindingBiological Process: protein folding; response to unfolded protein
