The DNAJC3 Polyclonal Antibody (PAC014815) is a valuable tool for researchers studying DNAJC3, a heat shock protein implicated in protein folding and ER stress responses. Produced in rabbits, this antibody exhibits high reactivity with human samples and is specifically validated for use in Western blot applications. By binding to the DNAJC3 protein, this antibody allows for precise detection and analysis in a variety of cell types, making it an ideal choice for studies in molecular biology and cellular stress pathways.
DNAJC3, also known as P58IPK, plays a crucial role in regulating protein folding and ER stress, which are essential processes for maintaining cellular homeostasis. Its involvement in these pathways makes it a key target for research into diseases such as cancer, neurodegenerative disorders, and metabolic syndromes. Understanding the function of DNAJC3 is essential for developing therapies aimed at modulating cellular stress responses and potentially treating a range of diseases linked to aberrant protein folding.
Antibody Name:
DNAJC3 Antibody (PACO14815)
Antibody SKU:
PACO14815
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:1000-1:5000, IHC:1:25-1:100
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Fusion protein of human DNAJC3
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using PACO14815(DNAJC3 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200).
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using PACO14815(DNAJC3 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200).
Background:
This gene encodes a protein with multiple tetratricopeptide repeat (TPR) motifs as well as the highly conserved J domain found in DNAJ chaperone family members. It is a member of the tetratricopeptide repeat family of proteins and acts as an inhibitor of the interferon-induced, dsRNA-activated protein kinase (PKR).
Synonyms:
DnaJ (Hsp40) homolog, subfamily C, member 3
UniProt Protein Function:
Involved in the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Acts as a negative regulator of the EIF2AK4/GCN2 kinase activity by preventing the phosphorylation of eIF-2-alpha at 'Ser-52' and hence attenuating general protein synthesis under ER stress, hypothermic and amino acid starving stress conditions (). Co-chaperone of HSPA8/HSC70, it stimulates its ATPase activity. May inhibit both the autophosphorylation of EIF2AK2/PKR and the ability of EIF2AK2 to catalyze phosphorylation of the EIF2A. May inhibit EIF2AK3/PERK activity.
UniProt Protein Details:
NCBI Summary:
This gene encodes a protein with multiple tetratricopeptide repeat (TPR) motifs as well as the highly conserved J domain found in DNAJ chaperone family members. It is a member of the tetratricopeptide repeat family of proteins and acts as an inhibitor of the interferon-induced, dsRNA-activated protein kinase (PKR). [provided by RefSeq, Jul 2010]
Endoplasmic reticulum DNA J domain-containing protein 6; ER-resident protein ERdj6; ERdj6; Interferon-induced, double-stranded RNA-activated protein kinase inhibitor; Protein kinase inhibitor of 58 kDa; Protein kinase inhibitor p58
