The DNAJA3 Polyclonal Antibody (CAB7030) is a valuable tool for researchers investigating DNAJA3, a member of the heat shock protein 40 (HSP40) family known for its chaperone activity in protein folding and assembly processes. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blotting applications. By binding specifically to the DNAJA3 protein, this antibody enables the detection and analysis of DNAJA3 expression in various cell types, making it an ideal reagent for studies in molecular biology and protein science.
DNAJA3 plays a crucial role in maintaining protein homeostasis and preventing protein misfolding, making it essential for proper cellular function and survival. Its involvement in protein quality control processes makes DNAJA3 a promising target for research into neurodegenerative diseases, cancer, and other protein misfolding disorders. By studying the function and regulation of DNAJA3, researchers can gain valuable insights into the mechanisms underlying these diseases and potentially identify new therapeutic strategies for targeting protein folding defects.
Product Name:
DNAJA3 Rabbit Polyclonal Antibody
SKU:
CAB7030
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 221-480 of human DNAJA3 (NP_005138.3).
This gene encodes a member of the DNAJ/Hsp40 protein family. DNAJ/Hsp40 proteins stimulate the ATPase activity of Hsp70 chaperones and play critical roles in protein folding, degradation, and multimeric complex assembly. The encoded protein is localized to mitochondria and mediates several cellular processes including proliferation, survival and apoptotic signal transduction. The encoded protein also plays a critical role in tumor suppression through interactions with oncogenic proteins including ErbB2 and the p53 tumor suppressor protein. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Purification Method:
Affinity purification
Gene ID:
9093
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using DNAJA3 antibody (CAB7030) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
