The DNAJA1 Polyclonal Antibody (CAB11602) is a specialized tool for research involving DNAJA1, a protein chaperone known for its role in protein folding and quality control. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the DNAJA1 protein, this antibody enables researchers to detect and analyze DNAJA1 in a variety of cell types, making it an invaluable tool for studies in protein folding mechanisms and cellular stress response.DNAJA1, a member of the DNAJ/HSP40 family of molecular chaperones, is essential for maintaining protein homeostasis within cells by assisting in the folding of newly synthesized proteins and helping to refold misfolded proteins under conditions of cellular stress.
Its critical role in protein quality control processes makes DNAJA1 a key player in various cellular functions, including the response to heat shock and other environmental stressors. Research into the mechanisms by which DNAJA1 operates is vital for understanding cellular protein quality control and its implications for disease, such as neurodegenerative disorders and cancer.By utilizing the DNAJA1 Polyclonal Antibody (CAB11602), researchers can delve deeper into the functions and regulatory mechanisms of DNAJA1, shedding light on its importance in cellular homeostasis and its potential as a therapeutic target in diseases involving protein misfolding and dysfunction.
Product Name:
DNAJA1 Rabbit Polyclonal Antibody
SKU:
CAB11602
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 178-397 of human DNAJA1 (NP_001530.1).
This gene encodes a member of the DnaJ family of proteins, which act as heat shock protein 70 cochaperones. Heat shock proteins facilitate protein folding, trafficking, prevention of aggregation, and proteolytic degradation. Members of this family are characterized by a highly conserved N-terminal J domain, a glycine/phenylalanine-rich region, four CxxCxGxG zinc finger repeats, and a C-terminal substrate-binding domain. The J domain mediates the interaction with heat shock protein 70 to recruit substrates and regulate ATP hydrolysis activity. In humans, this gene has been implicated in positive regulation of virus replication through co-option by the influenza A virus. Several pseudogenes of this gene are found on other chromosomes.
Purification Method:
Affinity purification
Gene ID:
3301
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type(WT) and DNAJA1 knockout (KO) 293T(KO) cells, using [KO Validated] DNAJA1 Rabbit pAb (CAB11602) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
