The DNAAF1 Polyclonal Antibody (CAB16593) is specifically designed for research involving DNAAF1, a protein implicated in cilia formation and function. The antibody, generated in rabbits, demonstrates high reactivity with human samples and is validated for use in Western blot applications. By binding to the DNAAF1 protein, researchers can detect and analyze its presence in various cell types, making it an essential tool for studies in cell biology and genetic disorders related to cilia dysfunction.DNAAF1, also known as dynein axonemal intermediate chain 1, plays a crucial role in the assembly and function of dynein motors within cilia, which are hair-like structures found on the surface of many cell types.
Dysfunction of DNAAF1 has been linked to various ciliopathies, including respiratory disorders and infertility. Understanding the function of DNAAF1 is vital for uncovering the mechanisms behind these conditions and developing potential therapies to target cilia-related diseases.Overall, the DNAAF1 Polyclonal Antibody provides researchers with a valuable tool to study the role of DNAAF1 in cilia biology and its implications for human health. Its high specificity and sensitivity make it an ideal choice for investigations into ciliopathies and related disorders.
Product Name:
DNAAF1 Rabbit Polyclonal Antibody
SKU:
CAB16593
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 516-725 of human DNAAF1 (NP_848547.4).
Cell projection, Cytoplasm, cilium, cytoskeleton, spindle pole.
Calculated MW:
80kDa
Observed MW:
80kDa
The protein encoded by this gene is cilium-specific and is required for the stability of the ciliary architecture. It is involved in the regulation of microtubule-based cilia and actin-based brush border microvilli. Mutations in this gene are associated with primary ciliary dyskinesia-13. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
123872
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from rat testis, using DNAAF1 Rabbit pAb (CAB16593) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
