DNA polymerase eta Rabbit Polyclonal Antibody (CAB1833)
The DNA Polymerase Eta Polyclonal Antibody (CAB1833) is a valuable tool for researchers studying DNA replication and repair processes. This antibody, generated in rabbits, specifically targets DNA polymerase Eta, a specialized enzyme involved in translesion synthesis that allows for the bypass of DNA lesions during replication. With high reactivity towards human samples, this antibody is perfect for use in Western blot applications, enabling the detection and analysis of DNA polymerase Eta in various cell types. Its specificity makes it an ideal choice for research in fields such as genetics, molecular biology, and cancer biology, where understanding DNA repair mechanisms is crucial.
DNA polymerase Eta plays a critical role in maintaining genome stability and preventing mutations by accurately replicating DNA across damaged regions. Dysfunction of this enzyme has been implicated in various diseases, including cancer and genetic disorders. By utilizing this antibody, researchers can gain valuable insights into the functions of DNA polymerase Eta and its impact on human health, ultimately leading to advancements in the development of targeted therapies.
Product Name:
DNA polymerase eta Rabbit Polyclonal Antibody
SKU:
CAB1833
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 322-520 of human DNA polymerase eta (NP_006493.1).
This gene encodes a member of the Y family of specialized DNA polymerases. It copies undamaged DNA with a lower fidelity than other DNA-directed polymerases. However, it accurately replicates UV-damaged DNA; when thymine dimers are present, this polymerase inserts the complementary nucleotides in the newly synthesized DNA, thereby bypassing the lesion and suppressing the mutagenic effect of UV-induced DNA damage. This polymerase is thought to be involved in hypermutation during immunoglobulin class switch recombination. Mutations in this gene result in XPV, a variant type of xeroderma pigmentosum. Several transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
5429
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using DNA polymerase eta Rabbit pAb (CAB1833) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
