DNA Ligase IV Rabbit Monoclonal Antibody (CAB11432)
The DNA Ligase IV Rabbit Monoclonal Antibody is an essential tool for researchers studying DNA repair mechanisms and genetic stability. This highly specific antibody binds to DNA Ligase IV, an enzyme involved in the final step of non-homologous end joining (NHEJ) pathway, which repairs double-strand breaks in DNA.This antibody, raised in rabbits, is validated for use in various applications including Western blotting, immunofluorescence, and immunoprecipitation. Its high affinity and specificity for DNA Ligase IV make it a reliable tool for detecting and quantifying the enzyme in a wide range of sample types, including cell lysates and tissue sections.
Understanding the role of DNA Ligase IV in DNA repair processes is critical for advancing research in cancer biology, genomics, and therapeutic development. By using this antibody, researchers can gain valuable insights into the mechanisms of DNA repair and identify potential targets for improving cancer treatment and genetic therapies.
Product Name:
DNA Ligase IV Rabbit Monoclonal Antibody
SKU:
CAB11432
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 504-685 of human DNA Ligase IV (P49917).
The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed.
Purification Method:
Affinity purification
Gene ID:
3981
Clone Number:
ARC0595
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using DNA Ligase IV Rabbit mAb (CAB11432) at 1：1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
