DNA Ligase IV Rabbit Monoclonal Antibody (CAB11432)
- SKU:
- CAB11432
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Monoclonal Antibody
- Research Area:
- Cell Cycle
Frequently bought together:
Description
Product Name: | DNA Ligase IV Rabbit Monoclonal Antibody |
SKU: | CAB11432 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 504-685 of human DNA Ligase IV (P49917). |
Sequence: | SRVG SGCT MKEL YDLG LKLA KYWK PFHR KAPP SSIL CGTE KPEV YIEP CNSV IVQI KAAE IVPS DMYK TGCT LRFP RIEK IRDD KEWH ECMT LDDL EQLR GKAS GKLA SKHL YIGG DDEP QEKK RKAA PKMK KVIG IIEH LKAP NLTN VNKI SNIF EDVE FCVM SGTD SQPK PDLE NRIA EF |
Tested Applications: | WB ELISA |
Recommended Dilution: | WB,1:500 - 1:2000 |
Synonyms: | LIG4S; DNA Ligase IV |
Positive Sample: | HeLa,MCF7,BxPC-3 |
Conjugate: | Unconjugated |
Cellular Localization: | Nucleus. |
Calculated MW: | 104kDa |
Observed MW: | 100kDa |
The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed.
Purification Method: | Affinity purification |
Gene ID: | 3981 |
Clone Number: | ARC0595 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3. |