DNA Ligase IV Rabbit Polyclonal Antibody (CAB1743)
- SKU:
- CAB1743
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Frequently bought together:
Description
Product Name: | DNA Ligase IV Rabbit Polyclonal Antibody |
SKU: | CAB1743 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human,Mouse,Rat |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human DNA Ligase IV (NP_002303.2). |
Sequence: | MAAS QTSQ TVAS HVPF ADLC STLE RIQK SKGR AEKI RHFR EFLD SWRK FHDA LHKN HKDV TDSF YPAM RLIL PQLE RERM AYGI KETM LAKL YIEL LNLP RDGK DALK LLNY RTPT GTHG DAGD FAMI AYFV LKPR CLQK GSLT IQQV NDLL DSIA SNNS AKRK DLIK KSLL QLIT QSSA LEQK WLIR MIIK DLKL GVSQ QTIF SVFH ND |
Tested Applications: | WB IHC-P ELISA |
Recommended Dilution: | WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 |
Synonyms: | LIG4S; DNA Ligase IV |
Positive Sample: | PC-3 |
Conjugate: | Unconjugated |
Cellular Localization: | Nucleus. |
Calculated MW: | 104kDa |
Observed MW: | 110kDa |
The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed.
Purification Method: | Affinity purification |
Gene ID: | 3981 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |