DNA Ligase IV Rabbit Polyclonal Antibody (CAB1743)
The DNA Ligase 4 Polyclonal Antibody (CAB1743) is a valuable tool for researchers studying DNA repair mechanisms and genetic stability. This antibody, generated in rabbits, specifically targets DNA Ligase 4, an essential enzyme involved in the ligation of DNA strand breaks during DNA replication and repair processes.DNA Ligase 4 is a key player in the non-homologous end joining (NHEJ) pathway, which repairs double-strand breaks in DNA. Dysregulation of this pathway can lead to genomic instability, making DNA Ligase 4 a potential target for cancer therapy and genetic disease research. The CAB1743 antibody is highly reactive with human samples and has been validated for use in Western blot applications, enabling precise detection and analysis of DNA Ligase 4 protein expression in various cell types.
This antibody is ideal for studies in molecular biology, genetics, and cancer research, providing researchers with a powerful tool to investigate the role of DNA Ligase 4 in DNA repair mechanisms and its potential as a therapeutic target for diseases characterized by genomic instability. By understanding the function and regulation of DNA Ligase 4, researchers can advance our knowledge of genetic stability and develop novel strategies for disease treatment and prevention.
Product Name:
DNA Ligase IV Rabbit Polyclonal Antibody
SKU:
CAB1743
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human DNA Ligase IV (NP_002303.2).
The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed.
Purification Method:
Affinity purification
Gene ID:
3981
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from PC-3 cells, using DNA Ligase IV Rabbit pAb (CAB1743) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
