The DLL1 Antibody (CAB14277) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity when detecting DLL1 in human samples, making it suitable for various research applications, including Western blot analysis.DLL1 is a transmembrane ligand that interacts with Notch receptors to regulate cell fate decisions, such as proliferation, differentiation, and apoptosis. Dysregulation of DLL1-Notch signaling has been implicated in various diseases, including cancer, neurodegenerative disorders, and developmental abnormalities.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
DLL1 Antibody
SKU:
CAB14277
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
DL1, Delta, DELTA1, NEDBAS, DLL1
Positive Sample:
Rat lung
Cellular Localization:
Apical Cell Membrane, Cell Junction, Membrane Raft, Single-Pass Type I Membrane Protein, Adherens Junction.
Calculated MW:
78kDa
Observed MW:
78kDa
DLL1 is a human homolog of the Notch Delta ligand and is a member of the delta/serrate/jagged family. It plays a role in mediating cell fate decisions during hematopoiesis. It may play a role in cell-to-cell communication.
Purification Method
Affinity purification
Gene ID
28514
RRID
AB_2761139
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Rat lung, using DLL1 Rabbit pAb (CAB14277) at 1:700 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Immunohistochemistry analysis of paraffin-embedded Rat pancreas using DLL1 Rabbit pAb (CAB14277) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
