The DLAT Rabbit Monoclonal Antibody (CAB8814) is a high-quality antibody designed for research involving DLAT, a key enzyme in the mitochondrial tricarboxylic acid cycle. This antibody, generated in rabbits, is highly specific and validated for use in various applications, including Western blot and immunohistochemistry.DLAT, also known as dihydrolipoamide S-acetyltransferase, plays a crucial role in energy production within the mitochondria by catalyzing the transfer of acetyl groups. Dysregulation of DLAT has been linked to various metabolic disorders, making it an important target for research in areas such as metabolism, diabetes, and mitochondrial diseases.
With its high reactivity and specificity, the DLAT Rabbit Monoclonal Antibody (CAB8814) enables accurate detection and analysis of DLAT in different samples and cell types. This antibody is a valuable tool for researchers studying mitochondrial function, metabolic pathways, and the role of DLAT in various diseases.
Product Name:
DLAT Rabbit Monoclonal Antibody
SKU:
CAB8814
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 548-647 of human DLAT (P10515).
This gene encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure. Mutations in this gene are also a cause of pyruvate dehydrogenase E2 deficiency which causes primary lactic acidosis in infancy and early childhood.
Purification Method:
Affinity purification
Gene ID:
1737
Clone Number:
ARC1314
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using DLAT Rabbit mAb (CAB8814) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1min.
