The DLAT Polyclonal Antibody (CAB6288) is a valuable tool for research involving DLAT, a crucial enzyme involved in energy metabolism. This polyclonal antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding to the DLAT protein, this antibody enables the detection and analysis of DLAT in various cell types, making it an essential component for studies in metabolism, energy production, and related diseases.DLAT, also known as dihydrolipoamide S-acetyltransferase, plays a vital role in the citric acid cycle and the production of ATP, the cell's main energy source.
Dysregulation of DLAT activity has been linked to metabolic disorders, neurodegenerative diseases, and cancer. Therefore, understanding the function and regulation of DLAT is essential for developing targeted therapies for these conditions.The DLAT Polyclonal Antibody is a valuable research tool that can provide insights into the complex mechanisms of energy metabolism and the potential therapeutic strategies for metabolic disorders and related diseases.
Product Name:
DLAT Rabbit Polyclonal Antibody
SKU:
CAB6288
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human DLAT (NP_001922.2).
This gene encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure. Mutations in this gene are also a cause of pyruvate dehydrogenase E2 deficiency which causes primary lactic acidosis in infancy and early childhood.
Purification Method:
Affinity purification
Gene ID:
1737
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using DLAT antibody (CAB6288) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
