The DLAT Polyclonal Antibody (CAB14530) is specifically designed for research involving DLAT, a key enzyme involved in the regulation of the pyruvate dehydrogenase complex, which plays a critical role in cellular energy metabolism. This antibody, produced in rabbits, offers high reactivity with human samples and has been validated for use in Western blot applications. By targeting the DLAT protein, this antibody enables the detection and analysis of DLAT in various cell types, making it an essential tool for researchers in the fields of metabolism and biochemistry.
DLAT is essential for converting pyruvate to acetyl-CoA in the mitochondria, a crucial step in the generation of ATP through the citric acid cycle. Dysregulation of DLAT activity has been associated with metabolic diseases such as diabetes and cancer, highlighting its importance in understanding cellular metabolism and energy production. By studying the function of DLAT, researchers can gain valuable insights into the mechanisms underlying metabolic disorders and potentially identify new therapeutic targets for intervention.
Product Name:
DLAT Rabbit Polyclonal Antibody
SKU:
CAB14530
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 87-270 of human DLAT (NP_001922.2).
This gene encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure. Mutations in this gene are also a cause of pyruvate dehydrogenase E2 deficiency which causes primary lactic acidosis in infancy and early childhood.
Purification Method:
Affinity purification
Gene ID:
1737
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates, using DLAT Rabbit pAb (CAB14530) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
