DLAT Rabbit Polyclonal Antibody (CAB14530)
- SKU:
- CAB14530
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
Product Name: | DLAT Rabbit Polyclonal Antibody |
SKU: | CAB14530 |
Size: | 20uL, 100uL |
Isotype: | IgG |
Host Species: | Rabbit |
Reactivity: | Human,Mouse,Rat |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 87-270 of human DLAT (NP_001922.2). |
Sequence: | SLPP HQKV PLPS LSPT MQAG TIAR WEKK EGDK INEG DLIA EVET DKAT VGFE SLEE CYMA KILV AEGT RDVP IGAI ICIT VGKP EDIE AFKN YTLD SSAA PTPQ AAPA PTPA ATAS PPTP SAQA PGSS YPPH MQVL LPAL SPTM TMGT VQRW EKKV GEKL SEGD LLAE IETD KATI GFEV QEEG |
Tested Applications: | WB IHC-P IF/ICC ELISA |
Recommended Dilution: | WB,1:500 - 1:2000 IHC-P,1:50 - 1:100 IF/ICC,1:50 - 1:200 |
Synonyms: | E2; PBC; DLTA; PDCE2; PDC-E2; DLAT |
Positive Sample: | K562,HeLa,MCF7 |
Conjugate: | Unconjugated |
Cellular Localization: | Mitochondrion matrix. |
Calculated MW: | 69kDa |
Observed MW: | 69kDa |
This gene encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure. Mutations in this gene are also a cause of pyruvate dehydrogenase E2 deficiency which causes primary lactic acidosis in infancy and early childhood.
Purification Method: | Affinity purification |
Gene ID: | 1737 |
Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |