The DKK4 Polyclonal Antibody (CAB7797) is a valuable tool for researchers studying the DKK4 protein, which is involved in regulating the Wnt signaling pathway, cell proliferation, and tumorigenesis. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the DKK4 protein, this antibody enables accurate detection and analysis in various cell types, making it an ideal choice for studies in cancer biology and cell signaling research.
DKK4 is a member of the Dickkopf family of proteins, known for their role in modulating Wnt signaling by binding to the LRP5/6 co-receptors. Dysregulation of the Wnt pathway, often associated with aberrant DKK4 expression, has been implicated in various diseases, including cancer and developmental disorders. Understanding the function of DKK4 and its interactions with other proteins is essential for unraveling its biological significance and exploring its potential as a therapeutic target in disease intervention.
Product Name:
DKK4 Rabbit Polyclonal Antibody
SKU:
CAB7797
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 19-224 of human DKK4 (NP_055235.1).
This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. Activity of this protein is modulated by binding to the Wnt co-receptor and the co-factor kremen 2.
Purification Method:
Affinity purification
Gene ID:
27121
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using DKK4 antibody (CAB7797) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
