The DKK1 Polyclonal Antibody (CAB2562) is a valuable tool for researchers studying DKK1, a secreted protein involved in the Wnt signaling pathway. This antibody, generated in rabbits, exhibits high reactivity with human samples and is suitable for use in Western blot applications. By specifically binding to DKK1, this antibody enables accurate detection and analysis of DKK1 protein levels in various types of cells, making it ideal for investigations in developmental biology, cancer research, and bone metabolism studies.DKK1 is a key regulator of the Wnt signaling pathway, which plays a critical role in embryonic development, tissue regeneration, and cancer progression.
Dysregulation of DKK1 expression has been linked to various diseases, making it a promising target for therapeutic interventions. By understanding the functions of DKK1 and its interactions within the Wnt signaling cascade, researchers can gain insights into the mechanisms underlying disease pathogenesis and develop targeted treatments to modulate Wnt signaling for therapeutic purposes.
Product Name:
DKK1 Rabbit Polyclonal Antibody
SKU:
CAB2562
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 32-266 of human DKK1 (NP_036374.1).
This gene encodes a member of the dickkopf family of proteins. Members of this family are secreted proteins characterized by two cysteine-rich domains that mediate protein-protein interactions. The encoded protein binds to the LRP6 co-receptor and inhibits beta-catenin-dependent Wnt signaling. This gene plays a role in embryonic development and may be important in bone formation in adults. Elevated expression of this gene has been observed in numerous human cancers and this protein may promote proliferation, invasion and growth in cancer cell lines.
Purification Method:
Affinity purification
Gene ID:
22943
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using DKK1 Rabbit pAb (CAB2562) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
