The Dio3 Polyclonal Antibody (CAB6900) is a vital tool for researchers studying the Dio3 protein, which plays a key role in thyroid hormone regulation and metabolism. This antibody, produced in rabbits, exhibits high reactivity towards human samples and has been thoroughly validated for use in Western blot applications. By binding specifically to the Dio3 protein, researchers can accurately detect and analyze its expression in various cell types, making it an essential component in studies related to endocrinology and metabolism.Dio3, also referred to as type 3 deiodinase, is an enzyme that regulates the levels of active thyroid hormones in the body, impacting metabolic processes and overall physiological functions.
Research into Dio3 is crucial for understanding thyroid hormone metabolism, as dysregulation of this enzyme has been linked to conditions such as hyperthyroidism and hypothyroidism. By studying Dio3 activity, researchers can gain insights into potential therapeutic targets for thyroid-related disorders and metabolic dysfunction.Overall, the Dio3 Polyclonal Antibody (CAB6900) serves as a valuable tool for researchers seeking to explore the role of Dio3 in thyroid hormone regulation and metabolic pathways, with potential implications for the development of novel treatments for thyroid disorders and metabolic diseases.
Product Name:
DIO3 Rabbit Polyclonal Antibody
SKU:
CAB6900
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 70-170 of human DIO3 (NP_001353.4).
Cell membrane, Endosome membrane, Single-pass type II membrane protein.
Calculated MW:
34kDa
Observed MW:
34kDa
The protein encoded by this intronless gene belongs to the iodothyronine deiodinase family. It catalyzes the inactivation of thyroid hormone by inner ring deiodination of the prohormone thyroxine (T4) and the bioactive hormone 3,3',5-triiodothyronine (T3) to inactive metabolites, 3,3',5'-triiodothyronine (RT3) and 3,3'-diiodothyronine (T2), respectively. This enzyme is highly expressed in pregnant uterus, placenta, fetal and neonatal tissues, and thought to prevent premature exposure of developing fetal tissues to adult levels of thyroid hormones. It regulates circulating fetal thyroid hormone concentrations, and thus plays a critical role in mammalian development. Knockout mice lacking this gene exhibit abnormalities related to development and reproduction, and increased activity of this enzyme in infants with hemangiomas causes severe hypothyroidism. This protein is a selenoprotein, containing the rare selenocysteine (Sec) amino acid at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal.
Purification Method:
Affinity purification
Gene ID:
1735
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using DIO3 antibody (CAB6900) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
