Anti-DIO2 Antibody (PACO58292)

Antibody Name:	DIO2 Antibody (PACO58292)
Antibody SKU:	PACO58292
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200
Species Reactivity:	Human
Immunogen:	Recombinant Human Type II iodothyronine deiodinase protein (74-222AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

IHC image of PACO58292 diluted at 1:400 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of A549 cells with PACO58292 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Background:	Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into T3 (3,5,3'-triiodothyronine). Essential for providing the brain with appropriate levels of T3 during the critical period of development.
Synonyms:	Type II iodothyronine deiodinase (EC 1.21.99.4) (5DII) (DIOII) (Type 2 DI) (Type-II 5'-deiodinase), DIO2, ITDI2 TXDI2

UniProt Protein Function:	DIO2: Responsible for the deiodination of T4 (3,5,3',5'- tetraiodothyronine) into T3 (3,5,3'-triiodothyronine). Essential for providing the brain with appropriate levels of T3 during the critical period of development. Belongs to the iodothyronine deiodinase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 1.97.1.10; Oxidoreductase; Membrane protein, integral Chromosomal Location of Human Ortholog: 14q24.2-q24.3 Cellular Component: membrane; plasma membrane Molecular Function:selenium binding; thyroxine 5'-deiodinase activity; ubiquitin protein ligase binding Biological Process: thyroid hormone generation; thyroid hormone metabolic process
NCBI Summary:	The protein encoded by this gene belongs to the iodothyronine deiodinase family. It catalyzes the conversion of prohormone thyroxine (3,5,3',5'-tetraiodothyronine, T4) to the bioactive thyroid hormone (3,5,3'-triiodothyronine, T3) by outer ring 5'-deiodination. This gene is widely expressed, including in thyroid, placenta, pituitary and brain. It is thought to be responsible for the 'local' production of T3, and thus important in influencing thyroid hormone action in these tissues. It has also been reported to be highly expressed in thyroids of patients with Graves disease, and in follicular adenomas. The intrathyroidal T4 to T3 conversion by this enzyme may contribute significantly to the relative increase in thyroidal T3 production in these patients. This protein is a selenoprotein containing the rare selenocysteine (Sec) amino acid at its active site, and may contain additional Sec residues. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2016]
UniProt Code:	Q92813
NCBI GenInfo Identifier:	172045839
NCBI Gene ID:	1734
NCBI Accession:	Q92813.4
UniProt Secondary Accession:	Q92813,Q6B0A3, Q9HCP8, Q9P1W4, Q9UDZ1, B9EGK0, G3V315
UniProt Related Accession:	Q92813
Molecular Weight:	34,704 Da
NCBI Full Name:	Type II iodothyronine deiodinase
NCBI Synonym Full Names:	deiodinase, iodothyronine, type II
NCBI Official Symbol:	DIO2
NCBI Official Synonym Symbols:	D2; 5DII; SelY; DIOII; TXDI2
NCBI Protein Information:	type II iodothyronine deiodinase
UniProt Protein Name:	Type II iodothyronine deiodinase
UniProt Synonym Protein Names:	5DII; DIOII; Type 2 DI; Type-II 5'-deiodinase
Protein Family:	Type II iodothyronine deiodinase
UniProt Gene Name:	DIO2
UniProt Entry Name:	IOD2_HUMAN

ELISA Kits

Human DIO2 / Type II iodothyronine deiodinase ELISA Kit

Human Type II iodothyronine deiodinase (DIO2) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)