The DIMT1 Polyclonal Antibody (CAB7589) is a valuable tool for researchers studying DIMT1, a key player in RNA modifications. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the DIMT1 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it ideal for studies in RNA biology and gene expression regulation.DIMT1, also known as dimerization and tRNA binding 1, plays a crucial role in catalyzing the formation of N6, N6-dimethyladenosine on tRNA, which is essential for proper translation and protein synthesis.
Understanding the function and regulation of DIMT1 is important for unraveling its impact on cellular processes like mRNA stability and RNA metabolism. Research on DIMT1 has implications for fields such as cancer biology, genetic diseases, and developmental biology, highlighting its relevance in advancing our understanding of RNA modifications and gene expression mechanisms.
Product Name:
DIMT1 Rabbit Polyclonal Antibody
SKU:
CAB7589
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-126 of human DIMT1 (NP_055288.1).
The protein encoded by this gene is a methyltransferase that is responsible for dimethylation of adjacent adenosines near the 18S rRNA decoding site. The encoded protein is essential for ribosome biogenesis, although its catalytic activity is not involved in the process. The yeast ortholog of this protein functions in the cytoplasm while this protein functions in the nucleus.
Purification Method:
Affinity purification
Gene ID:
27292
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using DIMT1 antibody (CAB7589) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
