The Dimethyl Histone H4 (K20) Polyclonal Antibody is a valuable tool for research involving histone modifications, specifically targeting dimethylated histone H4 at lysine 20. This antibody, raised in rabbits, exhibits high reactivity with human samples and is validated for use in various applications including Western blot and immunohistochemistry.Histone modifications play a crucial role in regulating gene expression, chromatin structure, and various cellular processes. Dimethylation of histone H4 at lysine 20 is known to be involved in gene silencing, DNA repair, and heterochromatin formation. By targeting this specific modification, researchers can gain insights into the epigenetic regulation of gene expression in different cell types and biological contexts.
The Dimethyl Histone H4 (K20) Polyclonal Antibody is a powerful tool for studying epigenetic modifications and their impact on cellular function and disease development. It enables researchers to detect and analyze dimethylated histone H4 at lysine 20, providing valuable information for studies in fields such as cancer research, stem cell biology, and developmental biology. Understanding the role of histone modifications like dimethylation of histone H4 at lysine 20 is essential for unraveling the complexity of gene regulation and identifying new therapeutic targets for various diseases.
Product Name:
DiMethyl-Histone H4-K20 Polyclonal Antibody
SKU:
CAB23888
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat,Other (Wide Range Predicted)
Immunogen:
A synthetic dimethylated peptide around K20 of human histone H4.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.
Purification Method:
Affinity purification
Gene ID:
8359
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates, using DiMethyl-Histone H4-K20 Rabbit pAb (CAB23888) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
