Anti-DiMethyl-Histone H4-K20 Antibody (CAB2371)
- SKU:
- CAB2371
- Product Type:
- Antibody
- Applications:
- WB
- IHC
- IF
- IP
- ChIP
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
Anti-DiMethyl-Histone H4-K20 Antibody (CAB2371)
The Dimethyl Histone H4 (K20) Polyclonal Antibody (CAB2371) is a valuable tool for researchers studying epigenetics and chromatin biology. This antibody specifically targets the dimethylated form of histone H4 at lysine 20, a modification linked to gene regulation and chromatin structure. Raised in rabbits, this antibody is highly specific to human samples and has been validated for use in various applications, including Western blotting and immunofluorescence.Histone modifications, such as dimethylation of histone H4 at lysine 20, play a crucial role in regulating gene expression and chromatin organization. Dysregulation of these modifications has been associated with various diseases, including cancer and developmental disorders.
By targeting this specific histone modification, researchers can investigate its impact on gene expression, cell differentiation, and disease progression.The Dimethyl Histone H4 (K20) Polyclonal Antibody offers a valuable tool for studying epigenetic mechanisms and chromatin dynamics in both normal and disease states. Its high reactivity and specificity make it an essential component of research projects exploring the role of histone modifications in gene regulation and cellular function.
| Antibody Name: | Anti-DiMethyl-Histone H4-K20 Antibody |
| Antibody SKU: | CAB2371 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF IP ChIP ChIPseq |
| Reactivity: | Human, Mouse, Rat, Other (Wide Range) |
| Host Species: | Rabbit |
| Immunogen: | A synthetic methylated peptide corresponding to residues surrounding K20 of human histone H4 |
| Application: | WB IHC IF IP ChIP ChIPseq |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200 ChIP 1:20 - 1:100 ChIPseq 1:20 - 1:100 |
| Reactivity: | Human, Mouse, Rat, Other (Wide Range) |
| Positive Samples: | HeLa, NIH/3T3, C6 |
| Immunogen: | A synthetic methylated peptide corresponding to residues surrounding K20 of human histone H4 |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 8370 |
| Uniprot: | P62805 |
| Cellular Location: | Chromosome, Nucleus |
| Calculated MW: | 11kDa |
| Observed MW: | 11KDa |
| Synonyms: | FO108, H4, H4/n, H4F2, H4FN, HIST2H4, Histone H4, HIST1H4A, HIST2H4A |
| Background: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy. |
| UniProt Protein Function: | Function: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| UniProt Protein Details: | Subunit structure: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Subcellular location: Nucleus. Chromosome. Post-translational modification: Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing. Ref.19 Ref.20 Ref.21 Ref.23 Ref.24 Ref.28Phosphorylated by PAK2 at Ser-48 (H4S47ph). This phosphorylation increases the association of H3.3-H4 with the histone chaperone HIRA, thus promoting nucleosome assembly of H3.3-H4 and inhibiting nucleosome assembly of H3.1-H4. Ref.28 Ref.36Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me). Ref.27 Ref.30Sumoylated, which is associated with transcriptional repression. Ref.22Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes. Ref.35 Sequence similarities: Belongs to the histone H4 family. Sequence caution: The sequence AAI28106.1 differs from that shown. Reason: Frameshift at position 3. |
| NCBI Summary: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the telomeric copy. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P62805 |
| NCBI GenInfo Identifier: | 77539758 |
| NCBI Gene ID: | 554313 |
| NCBI Accession: | NP_001029249.1 |
| UniProt Secondary Accession: | P62805,P02304, P02305, Q6DRA9, Q6FGB8, Q6NWP7, A2VCL0 |
| UniProt Related Accession: | P62805 |
| Molecular Weight: | 11,367 Da |
| NCBI Full Name: | histone H4 |
| NCBI Synonym Full Names: | histone cluster 2, H4b |
| NCBI Official Symbol: | HIST2H4B  |
| NCBI Official Synonym Symbols: | H4/o  |
| NCBI Protein Information: | histone H4; histone 2, H4b |
| UniProt Protein Name: | Histone H4 |
| Protein Family: | Histone |
| UniProt Gene Name: | HIST1H4A  |
| UniProt Entry Name: | H4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using DiMethyl-Histone H4-K20 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s. |
 | Dot-blot analysis of all sorts of methylation peptides using DiMethyl-Histone H4-K20 antibody (A2371). |
 | Immunohistochemistry of paraffin-embedded rat testis using DiMethyl-Histone H4-K20 antibody at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with IHC staining protocol. |
 | Immunohistochemistry of paraffin-embedded mouse testis using DiMethyl-Histone H4-K20 antibody at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with IHC staining protocol. |
 | Immunofluorescence analysis of 293T cells using DiMethyl-Histone H4-K20 antibody (A2371). Blue: DAPI for nuclear staining. |
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