The Dimethyl Histone H3 (K9) Polyclonal Antibody (CAB2359) is a valuable tool for researchers studying epigenetics and chromatin remodeling. This antibody is highly specific to dimethylated histone H3 (K9) and is raised in rabbits for optimal reactivity with human samples. Validated for use in techniques such as Western blotting, this antibody allows for precise detection and analysis of histone H3 (K9) methylation in various cell types.Histone modifications, including methylation of histone H3 at lysine 9, play a crucial role in regulating gene expression and chromatin structure.
The Dimethyl Histone H3 (K9) antibody enables researchers to investigate the role of this specific histone modification in processes such as transcriptional regulation, DNA repair, and various cellular pathways. With its ability to specifically recognize dimethylated histone H3 (K9), this antibody is essential for studies in epigenetics, cancer research, and developmental biology. Understanding the intricate mechanisms of histone modifications can provide insights into disease mechanisms and potential therapeutic targets for various disorders.
Product Name:
DiMethyl-Histone H3-K9 Rabbit Polyclonal Antibody
SKU:
CAB2359
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat,Other (Wide Range Predicted)
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human histone H3 (NP_003520.1).
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Purification Method:
Affinity purification
Gene ID:
8290/8350
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using DiMethyl-Histone H3-K9 Rabbit pAb (CAB2359) at 1:600 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
