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Anti-DiMethyl-Histone H3-K36 Antibody (CAB2365)

SKU:
CAB2365
Product Type:
Antibody
Applications:
WB
IHC
IF
IP
ChIP
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling
  • Epigenetics and Nuclear Signaling Antibodies 3 Anti-DiMethyl-Histone H3-K36 Antibody CAB2365
  • Epigenetics and Nuclear Signaling Antibodies 3 Anti-DiMethyl-Histone H3-K36 Antibody CAB2365
  • Epigenetics and Nuclear Signaling Antibodies 3 Anti-DiMethyl-Histone H3-K36 Antibody CAB2365
  • Epigenetics and Nuclear Signaling Antibodies 3 Anti-DiMethyl-Histone H3-K36 Antibody CAB2365
  • Epigenetics and Nuclear Signaling Antibodies 3 Anti-DiMethyl-Histone H3-K36 Antibody CAB2365
€139 - €419
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Description

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Anti-DiMethyl-Histone H3-K36 Antibody (CAB2365)

The Dimethyl-Histone H3 (K36) Polyclonal Antibody (CAB2365) is a vital tool for researchers studying histone modifications associated with gene expression regulation. This antibody, generated in rabbits, exhibits high specificity and reactivity with dimethylated histone H3 at lysine 36 in various species, making it ideal for use in immunofluorescence and chromatin immunoprecipitation (ChIP) assays.Histone modifications, such as methylation of lysine residues, play a crucial role in chromatin structure and gene transcription. The Dimethyl-Histone H3 (K36) antibody allows for the detection and analysis of this specific histone modification, providing valuable insights into epigenetic mechanisms governing gene expression.

Research involving histone modifications has significant implications for various fields, including cancer biology, developmental biology, and epigenetics. By using the CAB2365 antibody to study dimethylated histone H3 at lysine 36, scientists can deepen their understanding of how epigenetic changes influence gene regulation and disease development.

Antibody Name:Anti-DiMethyl-Histone H3-K36 Antibody
Antibody SKU:CAB2365
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF IP ChIP ChIPseq
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Host Species:Rabbit
Immunogen:A synthetic methylated peptide corresponding to residues surrounding K36 of human histone H3
Application:WB IHC IF IP ChIP ChIPseq
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200 ChIP 1:20 - 1:100 ChIPseq 1:20 - 1:100
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Positive Samples:HeLa, NIH/3T3
Immunogen:A synthetic methylated peptide corresponding to residues surrounding K36 of human histone H3
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:8290
Uniprot:Q16695
Cellular Location:Chromosome, Nucleus
Calculated MW:15kDa
Observed MW:17kDa
Synonyms:H3.4, H3/g, H3FT, H3t, HIST3H3, Histone H3, HIST1H3A
Background:Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
UniProt Protein Function:HIST3H3: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Expressed in testicular cells. Belongs to the histone H3 family.
UniProt Protein Details:

Protein type:DNA-binding

Chromosomal Location of Human Ortholog: 1q42

Cellular Component: nucleoplasm; nucleus

Molecular Function:protein binding; DNA binding; histone binding; protein heterodimerization activity

Biological Process: nucleosome assembly; negative regulation of protein oligomerization; protein heterotetramerization; telomere maintenance

NCBI Summary:Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008]
UniProt Code:Q16695
NCBI GenInfo Identifier:18202512
NCBI Gene ID:8290
NCBI Accession:Q16695.3
UniProt Secondary Accession:Q16695,Q6FGU4, B2R5K3,
UniProt Related Accession:Q16695
Molecular Weight:
NCBI Full Name:Histone H3.1t
NCBI Synonym Full Names:histone cluster 3, H3
NCBI Official Symbol:HIST3H3  
NCBI Official Synonym Symbols:H3t; H3.4; H3/g; H3FT  
NCBI Protein Information:histone H3.1t; H3/t; histone 3, H3; H3 histone family, member T
UniProt Protein Name:Histone H3.1t
UniProt Synonym Protein Names:H3/g
UniProt Gene Name:HIST3H3  
UniProt Entry Name:H31T_HUMAN
Western blot - DiMethyl-Histone H3-K36 Rabbit pAb Western blot analysis of extracts of various cell lines, using DiMethyl-Histone H3-K36 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
- DiMethyl-Histone H3-K36 Rabbit pAb Dot-blot analysis of all sorts of methylation peptides using DiMethyl-Histone H3-K36 antibody (A2365).
Immunohistochemistry - DiMethyl-Histone H3-K36 Rabbit pAb Immunohistochemistry of paraffin-embedded human breast using DiMethyl-Histone H3-K36 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry - DiMethyl-Histone H3-K36 Rabbit pAb Immunohistochemistry of paraffin-embedded rat testis using DiMethyl-Histone H3-K36 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry - DiMethyl-Histone H3-K36 Rabbit pAb Immunohistochemistry of paraffin-embedded mouse testis using DiMethyl-Histone H3-K36 antibody at dilution of 1:200 (40x lens).
Immunofluorescence - DiMethyl-Histone H3-K36 Rabbit pAb Immunofluorescence analysis of 293T cells using DiMethyl-Histone H3-K36 antibody (A2365). Blue: DAPI for nuclear staining.
Chromatin Immunoprecipitation - DiMethyl-Histone H3-K36 Rabbit pAb Chromatin immunoprecipitation analysis of γ-actin gene from 293 cell line, using DiMethyl-Histone H3-K36 antibody and rabbit IgG. P1, P2 and P3 were probes located on γ-actin gene as the schematic diagram illustrated. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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