The Dimethyl-Histone H3 (K27) Polyclonal Antibody (CAB2362) is a valuable tool for research involving epigenetics, specifically focusing on histone modifications related to gene expression regulation. Histone H3 lysine 27 methylation is a crucial epigenetic mark associated with transcriptional repression and chromatin compaction. The dimethylation of this residue is known to play a role in gene silencing and is often implicated in various diseases, including cancer and neurodevelopmental disorders.This polyclonal antibody, generated in rabbits, exhibits high specificity and sensitivity towards dimethylated histone H3 at lysine 27 in human samples. It has been validated for use in techniques such as Western blotting, immunofluorescence, and chromatin immunoprecipitation, allowing for the detection and visualization of this specific histone modification in various cell types and tissues.
By utilizing the Dimethyl-Histone H3 (K27) Polyclonal Antibody (CAB2362), researchers can gain valuable insights into the epigenetic regulation of gene expression and the impact of histone modifications on cellular processes. This antibody is an essential tool for studies investigating the role of histone modifications in health and disease, providing a deeper understanding of the intricate mechanisms governing gene expression control.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Purification Method:
Affinity purification
Gene ID:
8290/8350
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates, using DiMethyl-Histone H3-K27 Rabbit pAb (CAB2362) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
