The Dimethyl-Histone H3 (K14) Polyclonal Antibody (CAB5278) is a valuable tool for researchers studying histone modifications and their impact on gene expression regulation. This antibody, raised in rabbits, specifically targets the dimethylated form of Histone H3 at lysine 14, a key modification associated with transcriptional activation and chromatin accessibility.Validated for use in various applications, including Western blotting and immunofluorescence, this antibody allows for the detection and analysis of dimethyl-Histone H3 (K14) in a variety of cell types and experimental conditions. Its high reactivity with human samples makes it an ideal reagent for studies investigating epigenetic mechanisms in cancer, development, and other biological processes.
Understanding the role of histone modifications, such as dimethylation of Histone H3 at lysine 14, is essential for unraveling the complex regulatory networks that control gene expression and cellular function. By utilizing this antibody, researchers can gain valuable insights into the molecular mechanisms governing chromatin dynamics and epigenetic regulation, paving the way for novel therapeutic approaches in various disease contexts.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Purification Method:
Affinity purification
Gene ID:
8290/8350
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using DiMethyl-Histone H3-K14 Rabbit pAb (CAB5278) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
