Anti-DICER1 Antibody (CAB6021)

Antibody Name:	Anti-DICER1 Antibody
Antibody SKU:	CAB6021
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1200-1460 of human DICER1 (NP_085124.2).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:1000 IHC 1:50 - 1:200 IF 1:100 - 1:200 IP 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-251MG, SH-SY5Y

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1200-1460 of human DICER1 (NP_085124.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	NQLN YYKQ EIPV QPTT SYSI QNLY SYEN QPQP SDEC TLLS NKYL DGNA NKST SDGS PVMA VMPG TTDT IQVL KGRM DSEQ SPSI GYSS RTLG PNPG LILQ ALTL SNAS DGFN LERL EMLG DSFL KHAI TTYL FCTY PDAH EGRL SYMR SKKV SNCN LYRL GKKK GLPS RMVV SIFD PPVN WLPP GYVV NQDK SNTD KWEK DEMT KDCM LANG KLDE DYEE EDEE EESL MWRA PKEE ADYE DDFL EYDQ EHIR FIDN MLMG S
Gene ID:	23405
Uniprot:	Q9UPY3
Cellular Location:	Cytoplasm
Calculated MW:	92kDa/208kDa/218kDa
Observed MW:	250kDa

Synonyms:

DICER1, DCR1, Dicer, Dicer1e, HERNA, K12H4.8-LIKE, MNG1, RMSE2

Background:

This gene encodes a protein possessing an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus. The encoded protein functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	DICER1: a cytoplasmic type-III RNase that plays a central role in RNA interference (RNAi) pathways to produce the active small RNAs that represses gene expression. Possesses a DEAD box RNA helicase motif in its amino terminus and a dsRNA-binding motif in the carboxy terminus. Dicer, along with Ago2 andTRBP, are the main components of the RNA-induced silencing complex (RISC). Fragile X syndrome repeats form RNA hairpins that are cut by Dicer but do not activate the interferon-inducible protein kinase, PKR. Four alternatively spliced isoforms have been described.
UniProt Protein Details:	Protein type:Helicase; Cell development/differentiation; Ribonuclease; RNA-binding; EC 3.1.26.3; RNA processing Chromosomal Location of Human Ortholog: 14q32.13 Cellular Component: growth cone; axon; cytoplasm; dendrite; ER-Golgi intermediate compartment; nucleus; cytosol Molecular Function:endoribonuclease activity; protein binding; miRNA binding; ribonuclease III activity; metal ion binding; double-stranded RNA binding; deoxyribonuclease I activity; helicase activity; ATP binding Biological Process: zygote asymmetric cell division; regulation of neuron differentiation; regulation of cell cycle; regulation of oligodendrocyte differentiation; miRNA-mediated gene silencing, miRNA loading onto RISC; negative regulation of transcription from RNA polymerase II promoter; embryonic hindlimb morphogenesis; olfactory bulb interneuron differentiation; post-embryonic development; cardiac muscle cell development; pre-microRNA processing; angiogenesis; DNA fragmentation during apoptosis; defense response to virus; regulation of viral genome replication; spleen development; positive regulation of myelination; positive regulation of Schwann cell differentiation; hair follicle morphogenesis; inner ear receptor cell development; mRNA stabilization; RNA interference, targeting of mRNA for destruction; multicellular organism growth; stem cell maintenance; RNA interference, conversion of ds siRNA to ss siRNA; spindle assembly; spinal cord motor neuron differentiation; miRNA-mediated gene silencing, production of miRNAs; myelin formation in the peripheral nervous system; branching morphogenesis of a tube; cartilage development; gene expression; nerve development; cerebral cortex development; RNA interference, production of siRNA; reproductive structure development; neurite morphogenesis; lung development; myoblast cell differentiation involved in skeletal muscle regeneration Disease: Pleuropulmonary Blastoma; Goiter, Multinodular 1, With Or Without Sertoli-leydig Cell Tumors; Rhabdomyosarcoma, Embryonal, 2
NCBI Summary:	This gene encodes a protein possessing an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus. The encoded protein functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2010]
UniProt Code:	Q9UPY3
NCBI GenInfo Identifier:	257051056
NCBI Gene ID:	23405
NCBI Accession:	Q9UPY3.3
UniProt Secondary Accession:	Q9UPY3,O95943, Q9UQ02, A7E2D3, B3KRG4, E0AD28,
UniProt Related Accession:	Q9UPY3
Molecular Weight:	1922
NCBI Full Name:	Endoribonuclease Dicer
NCBI Synonym Full Names:	dicer 1, ribonuclease type III
NCBI Official Symbol:	DICER1
NCBI Official Synonym Symbols:	DCR1; MNG1; Dicer; HERNA; RMSE2; Dicer1e; K12H4.8-LIKE
NCBI Protein Information:	endoribonuclease Dicer; helicase MOI; Dicer1, Dcr-1 homolog; helicase with RNAse motif; dicer 1, double-stranded RNA-specific endoribonuclease
UniProt Protein Name:	Endoribonuclease Dicer
UniProt Synonym Protein Names:	Helicase with RNase motif; Helicase MOI
Protein Family:	Endoribonuclease
UniProt Gene Name:	DICER1
UniProt Entry Name:	DICER_HUMAN

	Western blot analysis of extracts of various cell lines, using DICER1 antibody (CAB6021) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min.
	Immunohistochemistry of paraffin-embedded Mouse brain using DICER1 Rabbit pAb (CAB6021) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Rat brain using DICER1 Rabbit pAb (CAB6021) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Human placenta using DICER1 Rabbit pAb (CAB6021) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of L929 cells using DICER1 Polyclonal Antibody (CAB6021) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-DICER1 Antibody (CAB15799)	Human Dicer ELISA Kit
DICER1 Antibody (PACO08839)	Human Endoribonuclease Dicer (DICER1) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)