DHX35 Antibody (PACO42174)

SKU:

PACO42174

Product Type:

Antibody

Reactivity:

Human

Mouse

Host Species:

Rabbit

Isotype:

IgG

Applications:

ELISA

IHC

Antibody Type:

Polyclonal Antibody

Conjugation:

Unconjugated

Description

Description

DatasheetMSDS

DHX35 Antibody (PACO42174)

The DHX35 Polyclonal Antibody (PAC042174) is a valuable tool for researchers studying the function of DHX35, a RNA helicase involved in RNA processing and gene expression regulation. This antibody, generated in rabbits, exhibits high specificity and sensitivity for detecting DHX35 in human samples, making it ideal for applications such as Western blotting.DHX35, also known as RNA helicase A, plays a crucial role in various cellular processes, including RNA splicing, translation, and RNA degradation.

Dysregulation of DHX35 has been linked to diseases such as cancer, viral infections, and neurodegenerative disorders, highlighting its importance in molecular biology and disease mechanisms.By using the DHX35 Polyclonal Antibody in their research, scientists can gain valuable insights into the role of DHX35 in cellular pathways and disease progression, ultimately paving the way for the development of targeted therapies and diagnostic tools.

RIMS4 Antibody (PACO64925)

€319

Product Code:

PACO64925

Antibody Type:

Polyclonal Antibody

Isotype:

IgG

Western Blot<br />
Positive WB detected in: Mouse heart tissue,Mouse kidney tissue<br />
All lanes: ompF antibody at 1:2000<br />
Secondary<br />
Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
Predicted band size: 57 kDa<br />
Observed band size: 57 kDa<br /> — Choose Options

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Ang4 Antibody (PACO64593)

€339

Product Code:

PACO64593

Antibody Type:

Polyclonal Antibody

Isotype:

IgG

Immunofluorescence staining of NIH/3T3 cells with PACO64869 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). — Choose Options

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OR13C8 Antibody (PACO64869)

€319

Product Code:

PACO64869

Antibody Type:

Polyclonal Antibody

Isotype:

IgG

Immunofluorescence staining of Hela cells with PACO64949 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). — Choose Options

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SIM1 Antibody (PACO64949)

€339

Product Code:

PACO64949

Antibody Type:

Polyclonal Antibody

Isotype:

IgG

Western Blot<br />
Positive WB detected in: Escherichia coli lysate<br />
All lanes: OTSB antibody at 1:2000<br />
Secondary<br />
Goat polyclonal to rabbit IgG at 1/50000 dilution<br />
Predicted band size: 30 kDa<br />
Observed band size: 30 kDa<br /> — Choose Options

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otsB Antibody (PACO64885)

€339

Product Code:

PACO64885

Antibody Type:

Polyclonal Antibody

Isotype:

IgG

IHC image of PACO64929 diluted at 1:300 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. — Choose Options

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RNF122 Antibody (PACO64929)

€319

Product Code:

PACO64929

Antibody Type:

Polyclonal Antibody

Isotype:

IgG

Immunofluorescence staining of SH-SY5Y cells with PACO64589-IF at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). — Choose Options

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AGO4 Antibody (PACO64589)

€319

Product Code:

PACO64589

Antibody Type:

Polyclonal Antibody

Isotype:

IgG

IHC image of PACO64729 diluted at 1:200 and staining in paraffin-embedded rat kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. — Choose Options

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Fn1 Antibody (PACO64729)

€339

Product Code:

PACO64729

Isotype:

IgG

IHC image of PACO64713 diluted at 1:200 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. — Choose Options

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FAM53A Antibody (PACO64713)

€339

Product Code:

PACO64713

Antibody Type:

Polyclonal Antibody

Isotype:

IgG

Antibody Name:	DHX35 Antibody (PACO42174)
Antibody SKU:	PACO42174
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:20-1:200
Species Reactivity:	Human, Mouse
Immunogen:	Recombinant Human Probable ATP-dependent RNA helicase DHX35 protein (1-300AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

Background:	May be involved in pre-mRNA splicing.
Synonyms:	Probable ATP-dependent RNA helicase DHX35 (EC 3.6.4.13) (DEAH box protein 35), DHX35, C20orf15 DDX35

UniProt Protein Function:	DHX35: May be involved in pre-mRNA splicing. Belongs to the DEAD box helicase family. DEAH subfamily.Protein type: RNA splicing; Spliceosome; Helicase; EC 3.6.4.13Chromosomal Location of Human Ortholog: 20q11.22-q12Molecular Function: ATP binding; ATP-dependent RNA helicase activityBiological Process: nuclear mRNA splicing, via spliceosome; RNA processing
UniProt Protein Details:
NCBI Summary:	DEAD box proteins characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The function of this gene product which is a member of this family, has not been determined. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jun 2010]
UniProt Code:	Q9H5Z1
NCBI GenInfo Identifier:	299829255
NCBI Gene ID:	60625
NCBI Accession:	NP_001177738.1
UniProt Secondary Accession:	Q9H5Z1,Q5THR0, Q9H4H7, Q9H6T6, A2RTX3, B4E0J0, F5GXM6
UniProt Related Accession:	Q9H5Z1
Molecular Weight:
NCBI Full Name:	probable ATP-dependent RNA helicase DHX35 isoform 2
NCBI Synonym Full Names:	DEAH-box helicase 35
NCBI Official Symbol:	DHX35
NCBI Official Synonym Symbols:	DDX35; C20orf15; KAIA0875
NCBI Protein Information:	probable ATP-dependent RNA helicase DHX35
UniProt Protein Name:	Probable ATP-dependent RNA helicase DHX35
UniProt Synonym Protein Names:	DEAH box protein 35
Protein Family:	Probable ATP-dependent RNA helicase
UniProt Gene Name:	DHX35
UniProt Entry Name:	DHX35_HUMAN

DHX35 Antibody (PACO42174)

Description

DHX35 Antibody (PACO42174)

Overview

Properties

Product Images

Additional Information

Related Products

	Western blot. All lanes: DHX35 antibody at 12µg/ml. Lane 1: Mouse kidney tissue. Lane 2: Mouse liver tissue. Lane 3: K562 whole cell lysate. Lane 4: Hela whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 79, 76 kDa. Observed band size: 79 kDa.
	Immunohistochemistry of paraffin-embedded human testis tissue using PACO42174 at dilution of 1:100.
	Immunohistochemistry of paraffin-embedded human brain tissue using PACO42174 at dilution of 1:100.

DHX35 Antibody (PACO42174)

Description

DHX35 Antibody (PACO42174)

Overview

Properties

Product Images

Additional Information

Related Products

Related Products

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