DHVD3 (1,25-Dihydroxyvitamin D3) ELISA Kit (UNES00012) (UNES00012)
- SKU:
- UNES00012
- Product Type:
- ELISA Kit
- Size:
- 96 Assays
- Sensitivity:
- 4.69pg/mL
- Range:
- 7.81-500pg/mL
- ELISA Type:
- Competitive
- Reactivity:
- Universal
- Sample Type:
- Serum, plasma and other biological fluids
Description
Product Name: | DHVD3 (1,25-Dihydroxyvitamin D3) ELISA Kit (UNES00012) |
SKU: | UNES00012 |
Target: | DHVD3 (1,25-Dihydroxyvitamin D3) (UNES00012) |
Size: | 96T |
Assay type: | Competitive-ELISA |
Assay time: | 2.0h |
Sensitivity: | 4.69 pg/mL |
Detection range: | 7.81-500 pg/mL |
Kit component: |
|
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with DHVD3. During the reaction, DHVD3 in the sample or standard competes with a fixed amount of DHVD3 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to DHVD3. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of DHVD3 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity: |
| ||||||||||||||||||||||||||||||||||||||||||
Recovery: |
| ||||||||||||||||||||||||||||||||||||||||||
Precision: |
|
Sample type &Sample volume: | serum, plasma and other biological fluids; 50μL |
Reproducibility: | Both intra-CV and inter-CV are < 10%. |
Application: | This ELISA kit applies to the in vitro quantitative determination of DHVD3 concentrations in serum, plasma and other biological fluids. |
Specificity: | This kit recognizes DHVD3 in samples. No significant cross-reactivity or interference between DHVD3 and analogues was observed. |