The 1,25-Dihydroxyvitamin D3 (DHVD3) ELISA Kit is a powerful tool for the accurate measurement of 1,25-dihydroxyvitamin D3 levels in biological samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.1,25-Dihydroxyvitamin D3, also known as calcitriol, is the active form of vitamin D in the body and plays a crucial role in calcium metabolism, bone health, and immune function.
Abnormal levels of 1,25-dihydroxyvitamin D3 have been linked to various disorders including osteoporosis, autoimmune diseases, and certain types of cancer, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.With its high performance and accuracy, the DHVD3 ELISA Kit is an essential tool for researchers and clinicians looking to investigate the role of 1,25-dihydroxyvitamin D3 in health and disease.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with DHVD3. During the reaction, DHVD3 in the sample or standard competes with a fixed amount of DHVD3 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to DHVD3. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of DHVD3 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-105
93-105
89-104
Average (%)
97
99
96
1:4
Range (%)
88-102
94-109
92-105
Average (%)
94
101
98
1:8
Range (%)
94-105
91-106
94-107
Average (%)
99
97
99
1:16
Range (%)
93-108
94-107
86-100
Average (%)
99
100
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-105
98
EDTA plasma (n=5)
88-101
94
Cell culture media (n=5)
88-103
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.85
42.69
171.01
25.28
43.72
154.82
Standard deviation
1.47
1.93
8.12
1.29
2.28
4.92
C V (%)
5.47
4.52
4.75
5.1
5.22
3.18
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of DHVD3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes DHVD3 in samples. No significant cross-reactivity or interference between DHVD3 and analogues was observed.
