The DHH Polyclonal Antibody (CAB13177) is a valuable tool for researchers studying the DHH protein, a member of the Hedgehog (Hh) family known for its role in embryonic development and cell differentiation. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in various applications, including Western blot.DHH, a secreted signaling molecule, plays a vital role in regulating cell growth, cell fate determination, and tissue patterning during development. Dysregulation of DHH signaling has been associated with various developmental disorders and cancers, highlighting its importance in biological processes.
The DHH Polyclonal Antibody binds specifically to the DHH protein, enabling researchers to detect and analyze its expression in a variety of cell types.By utilizing the DHH Polyclonal Antibody in their research, scientists can gain valuable insights into the function of DHH and its potential implications in diseases and developmental disorders. With its versatility and specificity, this antibody is a powerful tool for advancing our understanding of DHH biology and its therapeutic implications.
Product Name:
DHH Rabbit Polyclonal Antibody
SKU:
CAB13177
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 199-396 of human DHH (NP_066382.1).
This gene encodes a member of the hedgehog family. The hedgehog gene family encodes signaling molecules that play an important role in regulating morphogenesis. This protein is predicted to be made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the organism. Defects in this protein have been associated with partial gonadal dysgenesis (PGD) accompanied by minifascicular polyneuropathy. This protein may be involved in both male gonadal differentiation and perineurial development.
Purification Method:
Affinity purification
Gene ID:
50846
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using DHH Rabbit pAb (CAB13177) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
