The DHFR Polyclonal Antibody (CAB1607) is a valuable tool for research involving dihydrofolate reductase (DHFR), an enzyme essential for cellular metabolism and DNA synthesis. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the DHFR protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it ideal for studies in cancer research, pharmacology, and drug development.DHFR is a critical enzyme in the folate pathway, playing a key role in the synthesis of nucleotides needed for DNA replication and cell growth. Dysregulation of DHFR has been linked to cancer development and resistance to certain chemotherapy drugs, highlighting the importance of studying this enzyme in disease progression.
By targeting DHFR with this antibody, researchers can gain insights into the underlying mechanisms of cancer and potentially identify new therapeutic targets for intervention.Overall, the DHFR Polyclonal Antibody (CAB1607) offers researchers a reliable tool for studying the role of DHFR in cancer biology and drug resistance, advancing our understanding of molecular processes that drive disease progression. Its specificity and sensitivity make it a valuable asset in the quest for new treatments and interventions in oncology research.
Product Name:
DHFR Rabbit Polyclonal Antibody
SKU:
CAB1607
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-187 of human DHFR (NP_000782.1).
Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. Several transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
1719
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using DHFR antibody (CAB1607) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.
