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DHEA (Dehydroepiandrosterone) ELISA Kit (AEES00004)

SKU:
AEES00004
Product Type:
ELISA Kit
ELISA Type:
Competitive
Size:
96 Assays
Sensitivity:
0.10 ng/mL
Range:
0.16-10 ng/mL
Sample Type:
Serum, plasma and other biological fluids
€499
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:DHEA (Dehydroepiandrosterone) ELISA Kit
SKU:AEES00004
Target:DHEA (Dehydroepiandrosterone)
Size:96T
Assay type:Competitive-ELISA
Assay time:2.0h
Sensitivity:0.10 ng/mL
Detection range:0.16-10 ng/mL
Kit component:
ComponentQuantity (24 Assays)Quantity (96 Assays)Storage
Micro ELISA Plate (Dismountable)8 wells x 3 strips8 wells x 12 strips-20°C, 6 months
Reference Standard1 vial2 vials
Concentrated Biotinylated Detection Ab (100×)1 vial, 60 µL1 vial, 120 µL
Concentrated HRP Conjugate (100×)1 vial, 60 µL1 vial, 120 µL-20°C (shading light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL1 vial, 20 mL4°C, 6 months
Biotinylated Detection Ab Diluent1 vial, 14 mL1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL1 vial, 14 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL1 vial, 30 mL
Substrate Reagent1 vial, 10 mL1 vial, 10 mL4°C (shading light)
Stop Solution1 vial, 10 mL1 vial, 10 mL4°C
Plate Sealer5 pieces5 pieces
Product Description1 copy1 copy

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with DHEA. During the reaction, DHEA in the sample or standard competes with a fixed amount of DHEA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to DHEA. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of DHEA in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Linearity:
Serum (n=5)EDTA plasma (n=5)Cell culture media (n=5)
1:2Range (%)85-9696-11094-102
Average (%)9110297
1:4Range (%)85-9898-10791-98
Average (%)9010394
1:8Range (%)93-10396-10584-93
Average (%)9710090
1:16Range (%)96-10987-9996-107
Average (%)10195100
Recovery:
Sample TypeRange (%)Average Recovery (%)
Serum (n=5)96-103100
EDTA plasma (n=5)87-10295
Cell culture media (n=5)89-10195
Precision:
Intra-assay PrecisionInter-assay Precision
Sample123123
n2020.020.020.020.020.0
Mean (ng/mL)0.461.024.540.421.074.19
Standard deviation0.030.050.220.020.050.17
C V (%)6.524.94.854.764.674.06
Sample type &Sample volume:serum, plasma and other biological fluids; 50μL
Reproducibility:Both intra-CV and inter-CV are < 10%.
Application:This ELISA kit applies to the in vitro quantitative determination of DHEA concentrations in serum, plasma and other biological fluids.
Specificity:This kit recognizes DHEA in samples. No significant cross-reactivity or interference between DHEA and analogues was observed.

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