The DHCR24 Polyclonal Antibody (CAB5402) is a vital tool for research involving the DHCR24 protein, also known as 24-dehydrocholesterol reductase. This antibody, generated in rabbits, exhibits high reactivity towards human samples and is specifically validated for use in Western blot applications.DHCR24 is an enzyme that plays a crucial role in the cholesterol biosynthesis pathway, converting desmosterol to cholesterol. Dysregulation of DHCR24 has been linked to various diseases, including cancer, Alzheimer's, and Smith-Lemli-Opitz syndrome. By targeting DHCR24 with this polyclonal antibody, researchers can gain insight into its expression levels and activity in different cell types, aiding in the study of cholesterol metabolism and its implications in disease development.
This antibody is a valuable tool for researchers interested in exploring the role of DHCR24 in various biological processes, with potential applications in lipid metabolism research, cancer biology, and neurodegenerative diseases. Its high specificity and sensitivity make it an ideal choice for studies aiming to unravel the mechanisms underlying DHCR24 function and its involvement in disease pathways.
Product Name:
DHCR24 Rabbit Polyclonal Antibody
SKU:
CAB5402
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 227-516 of human DHCR24 (NP_055577.1).
This gene encodes a flavin adenine dinucleotide (FAD)-dependent oxidoreductase which catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. The protein contains a leader sequence that directs it to the endoplasmic reticulum membrane. Missense mutations in this gene have been associated with desmosterolosis. Also, reduced expression of the gene occurs in the temporal cortex of Alzheimer disease patients and overexpression has been observed in adrenal gland cancer cells.
Purification Method:
Affinity purification
Gene ID:
1718
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using DHCR24 antibody (CAB5402) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
