The DGKA Polyclonal Antibody (CAB13969) is a specialized research tool designed for the detection of DGKA, a key enzyme involved in lipid metabolism and signal transduction pathways. This antibody, produced in rabbits, exhibits high reactivity towards human samples and has been extensively validated for use in Western blot applications.DGKA, also known as diacylglycerol kinase alpha, plays a crucial role in cellular processes such as lipid signaling, membrane trafficking, and cell growth. Dysregulation of DGKA has been implicated in various diseases, including cancer, diabetes, and neurological disorders.
Understanding the function and regulation of DGKA is essential for unraveling the molecular mechanisms underlying these conditions.With its ability to specifically target and detect DGKA protein, this polyclonal antibody is a valuable tool for researchers investigating the role of DGKA in health and disease. Its versatility and reliability make it an ideal choice for studies in biochemistry, cell biology, and pharmacology, offering deeper insights into the complex interplay of lipid metabolism and signaling pathways.
Product Name:
DGKA Rabbit Polyclonal Antibody
SKU:
CAB13969
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human DGKA (NP_001336.2).
The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Several transcript variants encoding different isoforms have been identified for this gene.
Purification Method:
Affinity purification
Gene ID:
1606
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using DGKA antibody (CAB13969) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 20s.
