The DGKA Monoclonal Antibody (CAB22127) is a high-quality antibody developed for reliable detection and analysis of target proteins. This monoclonal antibody, developed using cutting-edge technology, specifically targets DGKA in human samples and is validated for use in various experimental applications such as immunofluorescence and flow cytometry.DGKA, also known as diacylglycerol kinase alpha, plays a crucial role in regulating lipid signaling and has been implicated in diseases such as cancer and neurodegenerative disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
DGKA Monoclonal Antibody
SKU:
CAB22127
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC55777
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
DAGK, DAGK1, DGK-alpha, DGKA
Positive Sample:
Jurkat, Mouse lung, Rat lung
Cellular Localization:
Cytosol, Plasma Membrane.
Calculated MW:
83kDa
Observed MW:
80kDa
The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Several transcript variants encoding different isoforms have been identified for this gene.
Purification Method
Affinity purification
Gene ID
1606
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using DGKA Rabbit mAb (CAB22127) at1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 60s.
Western blot analysis of lysates from Jurkat cells, using DGKA Rabbit mAb (CAB22127) at1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
