The DGCR8 Polyclonal Antibody (CAB18630) is specifically designed for research involving DGCR8, a key protein involved in microRNA biogenesis. This rabbit polyclonal antibody is highly reactive with human samples and has been validated for use in Western blot applications. By binding to the DGCR8 protein, this antibody enables precise detection and analysis in various cell types, making it an essential tool for studies in molecular biology and RNA processing research.DGCR8 plays a crucial role in the processing of microRNAs, small non-coding RNAs that regulate gene expression at the post-transcriptional level.
Dysregulation of microRNA biogenesis has been linked to various diseases, including cancer, cardiovascular disorders, and neurological conditions. Therefore, investigating the function of DGCR8 is essential for understanding the role of microRNAs in health and disease, as well as for developing potential therapeutic interventions targeting microRNA pathways. Overall, the DGCR8 Polyclonal Antibody is a valuable tool for researchers looking to explore the intricate mechanisms of microRNA processing and its implications in various physiological and pathological processes.
Product Name:
DGCR8 Rabbit Polyclonal Antibody
SKU:
CAB18630
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 180-340 of human DGCR8 (NP_073557.3).
WB,1:500 - 1:2000 IHC-P,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
Gy1; pasha; DGCRK6; C22orf12; DGCR8
Positive Sample:
HepG2,HeLa,293T,Mouse testis
Cellular Localization:
Nucleus, nucleolus.
Calculated MW:
86kDa
Observed MW:
110kDa
This gene encodes a subunit of the microprocessor complex which mediates the biogenesis of microRNAs from the primary microRNA transcript. The encoded protein is a double-stranded RNA binding protein that functions as the non-catalytic subunit of the microprocessor complex. This protein is required for binding the double-stranded RNA substrate and facilitates cleavage of the RNA by the ribonuclease III protein, Drosha. Alternate splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
54487
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using DGCR8 Rabbit pAb (CAB18630) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
