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DFFB Rabbit Polyclonal Antibody (CAB10110)

SKU:
CAB10110
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:DFFB Rabbit Polyclonal Antibody
SKU:CAB10110
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 109-338 of human DFFB (NP_004393.1).
Sequence:DEQA PQRQ RLLA DLLH NVSQ NIAA ETRA EDPP WFEG LESR FQSK SGYL RYSC ESRI RSYL REVS SYPS TVGA EAQE EFLR VLGS MCQR LRSM QYNG SYFD RGAK GGSR LCTP EGWF SCQG PFDM DSCL SRHS INPY SNRE SRIL FSTW NLDH IIEK KRTI IPTL VEAI KEQD GREV DWEY FYGL LFTS ENLK LVHI VCHK KTTH KLNC DPSR IYKP QTRL KRKQ PVRK RQ
Tested Applications:WB IHC-P ELISA
Recommended Dilution:WB,1:200 - 1:1000 IHC-P,1:50 - 1:200
Synonyms:CAD; CPAN; DFF2; DFF40; DFF-40; DFFB
Positive Sample:A-549,K-562,SW620,Mouse spleen,Mouse thymus
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:39kDa
Observed MW:39kDa

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of some of these variants has not been determined.

Purification Method:Affinity purification
Gene ID:1677
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of various cell lines, using DFFB antibody (CAB10110) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

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