The DFFA Polyclonal Antibody (CAB12431) is a highly specific tool designed for researchers studying the DFFA protein, a key player in apoptosis signaling pathways. This antibody, produced in rabbits, is suitable for use in Western blot applications and has been validated for its high reactivity with human samples.DFFA, also known as DNA fragmentation factor subunit alpha, is involved in DNA fragmentation during apoptosis, making it a vital target in cancer research and understanding cell death mechanisms. By binding to the DFFA protein, this polyclonal antibody allows for the detection and analysis of DFFA in various cell types, providing valuable insights into apoptosis regulation.
Researchers in the fields of molecular biology, cancer biology, and cell signaling will find this DFFA Polyclonal Antibody essential for their studies on apoptosis and the molecular mechanisms regulating cell death. Its specificity and reliability make it a valuable tool for unraveling the complexities of apoptosis pathways and developing targeted therapies for various diseases.
Product Name:
DFFA Rabbit Polyclonal Antibody
SKU:
CAB12431
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-331 of human DFFA (NP_004392.1).
Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
1676
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using DFFA antibody (CAB12431) at 1:1000 dilution. Jurkat cells were treated by Etoposide (25 uM) at 37℃ for 5 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
