The DET1 Homolog Polyclonal Antibody (CAB9974) is a specialized research tool designed for the study of DET1, a protein involved in light-mediated signaling pathways in plants. This antibody, produced in rabbits, is highly specific and reactive with plant samples, making it an essential tool for researchers studying photomorphogenesis and plant development.DET1 is a key regulator of light-dependent development processes in plants, including seed germination, seedling growth, and flowering. By targeting DET1 with this antibody in experiments such as Western blot analysis, researchers can gain valuable insights into the molecular mechanisms underlying light perception and response in plants.
With its high specificity and reliability in detecting DET1 protein, the DET1 Homolog Polyclonal Antibody (CAB9974) is an invaluable tool for plant biologists and researchers studying plant photobiology and molecular genetics. Its use can contribute to a better understanding of how plants sense and respond to light cues, with potential applications in agriculture, horticulture, and biotechnology.
Product Name:
DET1 Rabbit Polyclonal Antibody
SKU:
CAB9974
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 15-240 of human DET1 (NP_060466.2).
Enables ubiquitin ligase-substrate adaptor activity and ubiquitin protein ligase binding activity. Involved in positive regulation of proteasomal ubiquitin-dependent protein catabolic process; protein ubiquitination; and protein-containing complex assembly. Part of Cul4A-RING E3 ubiquitin ligase complex.
Purification Method:
Affinity purification
Gene ID:
55070
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using DET1 antibody (CAB9974) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 10s.
