The Desmoglein-3 Polyclonal Antibody (CAB8412) is a crucial tool for research involving Desmoglein-3, a cell adhesion molecule vital for maintaining the integrity of epithelial cells. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in various applications, including Western blot and immunohistochemistry. By specifically binding to the Desmoglein-3 protein, this antibody enables precise detection and analysis in different cell types, making it ideal for studies in dermatology, oncology, and autoimmune diseases.Desmoglein-3 is a key component of desmosomes, specialized structures that mediate strong cell-to-cell adhesion in epithelial tissues.
Dysregulation of Desmoglein-3 has been linked to skin disorders like pemphigus vulgaris, as well as certain types of cancer. Investigating the function and expression of Desmoglein-3 using this antibody can provide valuable insights into the pathogenesis of these conditions and potentially lead to the development of targeted therapies. Researchers interested in understanding the role of cell adhesion molecules in disease mechanisms will find the Desmoglein-3 Polyclonal Antibody an indispensable tool in their work.
Product Name:
Desmoglein 3 Rabbit Polyclonal Antibody
SKU:
CAB8412
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 749-999 of human Desmoglein 3 (NP_001935.2).
Cell junction, Cell membrane, Single-pass type I membrane protein, desmosome.
Calculated MW:
108kDa
Observed MW:
130kDa
This gene encodes a member of the desmoglein family and cadherin cell adhesion molecule superfamily of proteins. Desmogleins are calcium-binding transmembrane glycoprotein components of desmosomes, cell-cell junctions between epithelial, myocardial, and other cell types. The encoded preproprotein is proteolytically processed to generate the mature glycoprotein. This gene is present in a gene cluster with other desmoglein gene family members on chromosome 18. The encoded protein has been identified as the autoantigen of the autoimmune blistering disease pemphigus vulgaris.
Purification Method:
Affinity purification
Gene ID:
1830
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Desmoglein 3 antibody (CAB8412) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
