Anti-Desmoglein-1 Antibody (CAB9812)

Antibody Name:	Anti-Desmoglein-1 Antibody
Antibody SKU:	CAB9812
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 800-1049 of human DSG1 (NP_001933.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Rat
Positive Samples:	A-431, HeLa, 293T, BT-474

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 800-1049 of human DSG1 (NP_001933.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SPHF GTTT VISE STYP SGPG VLHP KPIL DPLG YGNV TVTE SYTT SDTL KPSV HVHD NRPA SNVV VTER VVGP ISGA DLHG MLEM PDLR DGSN VIVT ERVI APSS SLPT SLTI HHPR ESSN VVVT ERVI QPTS GMIG SLSM HPEL ANAH NVIV TERV VSGA GVTG ISGT TGIS GGIG SSGL VGTS MGAG SGAL SGAG ISGG GIGL SSLG GTAS IGHM RSSS DHHF NQTI GSAS PSTA RSRI TKYS TVQY SK
Gene ID:	1828
Uniprot:	Q02413
Cellular Location:	Cell junction, Cell membrane, Single-pass type I membrane protein, desmosome
Calculated MW:	42kDa/113kDa
Observed MW:	150kDa

Synonyms:

DSG1, CDHF4, DG1, DSG, EPKHE, EPKHIA, PPKS1, SPPK1

Background:

This gene encodes a member of the desmoglein protein subfamily. Desmogleins, along with desmocollins, are cadherin-like transmembrane glycoproteins that are major components of the desmosome. Desmosomes are cell-cell junctions that help resist shearing forces and are found in high concentrations in cells subject to mechanical stress. This gene is found in a cluster with other desmoglein family members on chromosome 18. The encoded protein has been identified as a target of auto-antibodies in the autoimmune skin blistering disease pemphigus foliaceus. Disruption of this gene has also been associated with the skin diseases palmoplantar keratoderma and erythroderma.

UniProt Protein Function:	DSG1: Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. Defects in DSG1 are the cause of palmoplantar keratoderma striate type 1 (SPPK1); also known as keratosis palmoplantaris striata I. SPPK1 is a dermatoligical disorder characterized by thickening of the skin on the palms and soles, and longitudinal hyperkeratotic lesions on the palms, running the length of each finger.
UniProt Protein Details:	Protein type:Cell adhesion; Membrane protein, integral Chromosomal Location of Human Ortholog: 18q12.1 Cellular Component: desmosome; internal side of plasma membrane; apical plasma membrane; integral to membrane; plasma membrane; intercellular junction; cytosol; lateral plasma membrane Molecular Function:toxin binding; gamma-catenin binding; calcium ion binding Biological Process: intercellular junction assembly; cell-cell adhesion; maternal process involved in pregnancy; protein stabilization; apoptosis; calcium-dependent cell-cell adhesion; response to progesterone stimulus; homophilic cell adhesion; cell structure disassembly during apoptosis Disease: Palmoplantar Keratoderma I, Striate, Focal, Or Diffuse; Erythroderma, Congenital, With Palmoplantar Keratoderma, Hypotrichosis, And Hyper-ige
NCBI Summary:	Desmosomes are cell-cell junctions between epithelial, myocardial and certain other cell types. Desmoglein 1 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. The protein encoded by this gene has been identified as the autoantigen of the autoimmune skin blistering disease pemphigus foliaceus. [provided by RefSeq, Jul 2008]
UniProt Code:	Q02413
NCBI GenInfo Identifier:	119703744
NCBI Gene ID:	1828
NCBI Accession:	NP_001933.2
UniProt Related Accession:	Q02413
Molecular Weight:	113,748 Da
NCBI Full Name:	desmoglein-1 preproprotein
NCBI Synonym Full Names:	desmoglein 1
NCBI Official Symbol:	DSG1
NCBI Official Synonym Symbols:	DG1; DSG; CDHF4; PPKS1; SPPK1
NCBI Protein Information:	desmoglein-1; DGI; cadherin family member 4; desmosomal glycoprotein 1; pemphigus foliaceus antigen
UniProt Protein Name:	Desmoglein-1
UniProt Synonym Protein Names:	Cadherin family member 4; Desmosomal glycoprotein 1; DG1; DGI; Pemphigus foliaceus antigen
Protein Family:	Desmoglein
UniProt Gene Name:	DSG1
UniProt Entry Name:	DSG1_HUMAN

	Western blot analysis of extracts of various cell lines, using DSG1 antibody (CAB9812) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded human trachea using DSG1 antibody (CAB9812) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of rat skin using DSG1 antibody (CAB9812) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
DSG1 Antibody (PACO02602)	Human Desmoglein 1 ELISA Kit
DSG1 Antibody (PACO07349)	Human Desmoglein-1 (DSG1) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)